[Skip to Content]
[Skip to Content Landing]
Other
April 1920

A STUDY OF BRAIN REPAIR IN THE RAT BY THE USE OF TRYPAN BLUEWITH SPECIAL REFERENCE TO THE VITAL STAINING OF THE MACROPHAGES

Author Affiliations

BALTIMORE

From the Department of Anatomy, Johns Hopkins Medical School and the Anatomical Laboratories, School of Medicine, University of Pittsburgh.

Arch NeurPsych. 1920;3(4):353-NP. doi:10.1001/archneurpsyc.1920.02180160002001
Abstract

Not a little light has been shed on certain of the phagocytic cells found in areas of aseptic inflammation by the use, in histophysiologic investigations, of the so-called "vital-dyes," and particularly by the employment of the azo dyes of the benzidin series. Tschaschin1 produced lesions of lymph glands, liver and spleen by burning with a red-hot needle, and introduced trypan blue, and other similar substances, into the circulating fluids of the animals during the period of repair. He showed that the mononuclear phagocytes, which throng the inflamed tissues around these lesions, rapidly became filled with large quantities of the dyestuff, held in the form of numberless minute granules or droplets. Other workers have noted similar phenomena, and it is generally recognized now that these mononuclear phagocytic cells, during a certain period of their existence at least, take up such dyes from the surrounding fluids with avidity, and store them

×