Cellular changes appearing at some stages of experimental acute anterior poliomyelitis have been recorded on several occasions.1 However, the changes that develop throughout the experimental disease have not been recorded, nor has the nonspecific arachnoiditis that follows intracerebral inoculation been taken into account.2 Therefore it seemed important to report the changes in the spinal fluid that occurred in over one hundred Macacus rhesus monkeys at the various stages of the disease and to correlate these changes with the clinical manifestations and various experimental types of the disease.
—The spinal fluid was obtained from the cisterna magna of animals anesthetized with ether. Only the findings of clear taps were considered. To the spinal fluid was added an equal amount of 10 per cent solution of glacial acetic acid colored well with methylene blue, and after the mixture had been agitated for five minutes the counts were
BRODIE M, WORTIS SB. EXPERIMENTAL POLIOMYELITISCYTOLOGIC STUDIES OF THE CEREBROSPINAL FLUID AND THE RESPIRATORY METABOLISM OF THE EXCISED SPINAL CORD AND BRAIN. Arch NeurPsych. 1934;32(6):1159-1172. doi:10.1001/archneurpsyc.1934.02250120036004