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June 1937

Theoretische und experimentelle Studien zur Methylenblaufärbung des Nervengewebes. Heft 1. Acta morphologica. Arbeiten aus dem morphologischen Laboratorium der Filiale des Institutes für experimentelle Medizin (WIEM) und dem Staatlichen anatomischen Institut in Gorkij (UdSSR.).

Arch NeurPsych. 1937;37(6):1468. doi:10.1001/archneurpsyc.1937.02260180248020

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Abstract

In this monograph, divided into three chapters, the author discusses in great detail the principles underlying the famous methylene blue (methylthionine chloride) staining method introduced by Ehrlich fifty years ago. It is also known as the vital staining method, for one of the prerequisites for successful staining laid down by Ehrlich was the living condition of the tissues. Schabadasch justly points out that the staining methods used generally for research or other purposes are for the most part empirical procedures. Theoretical considerations underlying the rationale of the staining methods are usually ignored. Such an attitude, of course, is a great detriment to science, for often only knowledge of the theoretical principles may lead the way to rendering a method workable, even in tissues to which ordinarily they are not applicable. The correctness of such views was splendidly proved by Schabadasch, who studied the principles underlying the methylene blue staining method

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