In pursuit of previously published studies,1 the problem presented itself whether a relationship can be established between substances in the blood having adrenergic and cholinergic properties and specific emotional reactions. Investigations were carried out on patients suffering from various psychopathologic reactions in which different emotions of varying intensity were demonstrable, and on members of the staff and medical students. All subjects were without demonstrable somatic disease.
The effect of samples of fresh whole blood on the contractions of an isolated strip of the rabbit duodenum was investigated by the following procedure: Healthy adult rabbits were killed by a blow on the head. The intestine was cut at the pylorus, and a strip approximately 10 cm. long was removed immediately and, with careful handling, was washed in Ringer-Tyrode solution. A section from 1 to 1.5 cm. long, attached to a recording lever, was suspended in about 180 cc.
DIETHELM O, DOTY EJ, MILHORAT AT. EMOTIONS AND ADRENERGIC AND CHOLINERGIC CHANGES IN THE BLOOD. Arch NeurPsych. 1945;54(2):110-115. doi:10.1001/archneurpsyc.1945.02300080038004