In view of reports indicating damage to the liver following the use of chlorpromazine * and our earlier observations3 indicating S35 - chlorpromazine to be rapidly accumulated in brain tissue, it was decided to study the pattern of accumulation of this new important therapeutic agent upon repeated administration.
Twenty-four adult male rats (150-200 gm.) were divided into four groups and given daily intraperitoneal injections of S35-chlorpromazine (50 mg/kg.). One group was killed each day, so that samples of animals having one, two, three, and four doses of chlorpromazine were obtained. The animals were killed by exsanguination, followed by perfusion with saline. The brains were carefully dissected into the important anatomical sections indicated below. The tissues, liver and brain, were separated into protein and lipid fractions by precipitating 1:10 aqueous homogenates (plasma was diluted with water) with 10 volumes of acetone-alcohol-ether mixture (1: 1:1). The protein precipitate was
WASE AW, CHRISTENSEN J, POLLEY E. The Accumulation of S35 -Chlorpromazine in Brain. AMA Arch NeurPsych. 1956;75(1):54-56. doi:10.1001/archneurpsyc.1956.02330190070005