There has probably been more research on the pathway of tetanus toxin to the central nervous system than on that of any other neurotropic agent. In spite of this, considerable controversy exists as to whether some element of the peripheral nerve or the vascular stream constitutes the primary route.
The present investigation was undertaken in the hope of resolving some of the problems relating to the communications between the periphery and the central nervous system. Although tetanus toxin was the only agent studied, the results may suggest likely routes for other neurotropic substances.
A highly inbred Sprague-Dawley strain of adult rats was used in all experiments. The minimum lethal dose (M. L. D., least amount of tetanus toxin which would kill all animals) was determined by Fedinec and Matzke1 to be 0.0015 ml/100 gm. of body weight of a 1:400 dilution of the toxin injected intramuscularly. The
FEDINEC AA, MATZKE HA. Course of a Neurotropic Toxin to the Central Nervous System. AMA Arch NeurPsych. 1959;81(2):148-153. doi:10.1001/archneurpsyc.1959.02340140014003