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Book and Media Reviews
October 3, 2007

Bone Marrow and Stem Cell Transplantation Renewing the Stuff of Life: Stem Cells, Ethics, and Public Policy

JAMA. 2007;298(13):1569-1574. doi:10.1001/jama.298.13.1571

This review concerns 2 books on stem cell transplantation: one on the molecular methods for various protocols and the other on public policy as it relates to embryonic stem cell transplantation. Both relate to different aspects of stem cell transplantation, from basic biology to public policy.

Bone Marrow and Stem Cell Transplantation is part of a continuing series in the Methods of Molecular Medicine handbooks. It has contributions from experts from around the world. The book is devoted to molecular biology protocols for a wide array of stem cell testing, not only for treatment of disease but primarily for research studies. The reference text is a compilation of protocols, starting with protocols for HLA typing including polymerase chain reaction (PCR) priming, sequence-specific oligonucleotide-primed PCR, and other sequencing-based methods. It includes protocols for detecting and identifying non-HLA polymorphisms, which only recently have become recognized as affecting transplantation outcomes. Other protocols relate to typing for epithelial cell receptors in relation to tissue regeneration, with emphasis on the emerging role of NOD2/CARD125 genes. Protocols are discussed for lymphoid and epithelial cell tracking as ways of determining chimerism of donor cells in host tissues, either for repopulation of a previously myeloablated bone marrow or for regeneration of injured tissue.

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