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Multiple Discipline Research Forum
March 7, 1964

Effect of Autologous Marrow Buffy Coat on Survival After Supralethal Irradiation

Author Affiliations


From the departments of surgery (Drs. Bloch, Longerbeam, Manax, Scott, and Lillehei) and radiology (Dr. Hilal), University of Minnesota School of Medicine. Dr. Bloch is a clinical trainee of the National Cancer Institute. Dr. Longerbeam is a Special Research Fellow of the United States Public Health Service. Dr. Lillehei is Markle Scholar in Academic Medicine and Associate Professor of Surgery.

JAMA. 1964;187(10):729-733. doi:10.1001/jama.1964.03060230057015

The PROTECTIVE EFFECT of autologous bone marrow used in the treatment of the pernicious effects of total body irradiation in dogs is well established.1,2 It is also firmly established that the efficacy of such treatment is directly proportional to the number of bone marrow cells infused into the irradiated dog.3 The procurement of sufficient marrow cells to obtain maximal protection begins to become a problem when supralethal doses of total body irradiation (600 to 1,000 roentgens) are used in the dog. For example, in our own experience, 2 to 3 billion marrow cells are usually sufficient to provide protection in the dog against total body irradiation of 500 r, while at least 5 billion marrow cells are needed when the dose of irradiation is increased to 600 to 1,000 r. The removal of large volumes of autologous marrow introduces the problem of hypovolemia, which adds to the morbidity

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