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Original Contributions
June 29, 1964

Fluorescent Antibody Diagnosis of Infections

Author Affiliations

Dallas; Anchorage, Alaska

Dr. Nelson is assistant professor of pediatrics and Miss Hempstead is a research technician at the Univerity of Texas Southwestern Medical School, Dallas. Mrs. Tanaka is a microbiologist and Mr. Pauls is chief of laboratories at the South Central Regional Laboratories, Alaska Division of Public Health, Anchorage. Mr. Pauls is now with the Department of Parasitology, School of Public Health, University of North Carolina, Chapel Hill.

JAMA. 1964;188(13):1121-1124. doi:10.1001/jama.1964.03060390023006

In areas without bacteriology laboratory facilities, diagnosis of certain infections can be made by the fluorescent antibody technique from specimens mailed to a central laboratory. An outbreak of respiratory illness occurred in a remote area of Alaska. Nasopharyngeal swab specimens applied to microscope slides were sent to laboratories in Anchorage, Alaska, and Dallas, Tex. Positive fluorescent antibody identification of Bordetella pertussis was made in 14 specimens by the Anchorage laboratory and confirmed in nine specimens in Dallas. Good correlation was obtained in nine cases of diarrheal disease studied by fluorescent antibody staining of rectal swab specimens for enteropathogenic serotypes of Escherichia coli.