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March 2, 1963

Fat Absorption and Acid Phosphatase Activity in Intestinal Epithelium of Mice

Author Affiliations

New York City

From the Department of Pathology, The Mount Sinai Hospital.

JAMA. 1963;183(9):761-764. doi:10.1001/jama.1963.63700090007013b
Abstract

WHATEVER has been learned about the occurrence and location of acid phosphatase in intestinal epithelium has not been incorporated into concepts of the physiology of absorption. Formerly, acid phosphatase has been located mainly in the Golgi zone, and thus its existence was buried in the complex and controversial problem of the Golgi apparatus. The three objectives of this communication are to demonstrate by improved histochemical techniques the location of acid phosphatase activity in the small intestine of the mouse, to reveal its decrease that follows forced feeding of oil, and to identify with the electron microscope structures that are associated with this enzyme activity.

Material and Methods  Mice of both sexes, weighing about 30 gm, were killed by distortion of the neck after 24 to 36 hr of fasting or at various intervals from 30 min to 6 hr after being fed 0.2 ml corn oil through a stomach tube.

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