To the Editor In the JAMA Clinical Challenge presenting an infant with neonatal infection with herpes simplex virus (HSV), Dr Ladizinski and colleagues1 recommended performing rapid direct fluorescence antibody (DFA) testing from a vesicle to establish the diagnosis. Ladizinski et al1 stated that DFA should be chosen over alternative diagnostic techniques, including viral cultures and polymerase chain reaction (PCR) assays, because DFA provides results most rapidly.
Rojek NW, Norton SA. Diagnosis of Neonatal Infection With Herpes Simplex Virus. JAMA. 2014;311(5):527-528. doi:10.1001/jama.2013.285414