Letters Section Editor: Robert M. Golub,
MD, Senior Editor.
To the Editor: Dr Gravett et al1 studied
33 samples of human amniotic fluid in an attempt to relate biomarkers to intra-amniotic
infection (IAI). Surface-enhanced laser desorption-ionization/time-of-flight
(SELDI-TOF) mass spectrometry identified possible biomarker peaks in preterm
intra-amniotic fluid in the 3- to 5-kDa and 11- to 12-kDa regions. The authors
supported their conclusions using tryptic digests subject to liquid chromatography–tandem
mass spectrometry analysis. They argued that calgranulin B and a fragment
of insulinlike growth factor binding protein 1 (IGFBP-1) are biomarkers of
IAI. Several aspects of this preliminary communication remain unclear.
Buhimschi IA, Buhimschi CS, Christner R, Norwitz E, Weiner CP. Proteomic Profiling and Intra-amniotic Infection. JAMA. 2004;292(19):2339-2340. doi:10.1001/jama.292.19.2338-a