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After reading the work done by Gaffky, Durham, Gruber and others with the Bacillus typhosus, noting the dilutions to a greater or less degree, for the purpose of lessening the inhibitive power of the serum, the idea came to me that the blood-clot was the essential part in the growing of organisms from the blood. Accordingly after doing the Widal agglutination test in a case that was not a clear enteric, I was prompted, after the removal of the serum from the Widal tube, to put the small remaining clot in a tube of bouillon. This I incubated for twelve hours and then examined by means of the hanging drop. I noted many organisms morphologically characteristic of the Bacillus typhosus. From this tube I inoculated a tube of litmus milk, one of agar slant, one of potato and a stab of glucose agar and incubated over night. On examination the
SHOUDY LA. PRELIMINARY REPORT OF WORK ON CLOTCULTURE. JAMA. 1910;55(2):126. doi:10.1001/jama.1910.04330020030011