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December 10, 1910

THE CULTIVATION OF TISSUES OF THE CHICK-EMBRYO OUTSIDE THE BODY

Author Affiliations

NEW HAVEN, CONN.

From the Sheffield Biological Laboratory, Yale University.

JAMA. 1910;55(24):2057-2058. doi:10.1001/jama.1910.04330240035009
Abstract

In 1907 Harrison described briefly before the Society of Experimental Biology and Medicine1 a method which he had found successful for the growing of certain tissues of the frog embryo outside the body. Essentially the method consisted in dissecting the central nervous system, myotomes and skin of frog embryos free from the surrounding tissues and transplanting them to a drop of lymph taken from the lymph-sac of an adult frog and contained within a hollow slide. The lymph immediately clots about the tissue elements into a loose fibrin network. Harrison watched the growth of the axis-cylinder processes of nerves and the proliferation and wandering of epithelial and connective-tissue cells within this matrix. He further observed striated embryonic muscle cells to become differentiated from the preexisting cells of the myotomes. In other words, he was able to observe a certain amount of differentiation of the tissues over a short period of

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