October 4, 1913

Serodiagnosis of Pregnancy

Author Affiliations

Clarksburg, W. Va.

JAMA. 1913;61(14):1313. doi:10.1001/jama.1913.04350150069027

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To the Editor:  —The main difficulty so far in the serodiagnosis of pregnancy has been the danger of bacterial contamination. I have had no trouble since using the following precautions: 1. The prepared placenta is kept in distilled water containing a few drops of chloroform and a layer of toluol. Just before use a small piece of placenta is removed and boiled in a small quantity of distilled water. This insures sterility and also removes any chloroform present.2. When ready the dialyzers are lowered into ordinary hydrometer jars without lips. After toluol has been added to the dialyze and the water, the jar is tightly corked. There is almost no evaporation.3. Immediately after use the thimbles are thoroughly washed in running tap-water and drained by inverting them on a sheet of filter-paper. Just before use they are again washed and boiled in distilled water for three minutes. [Comment

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