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The length of time and the complicated technic, as formerly required for preparation of the eosinate of methylene blue, led me to devise a method by which this stain may be prepared ready for use in less than an hour.
The stain depends on the formation of methylene azure and methylene violet in the alkaline methylic alcohol eosin methylene blue solution. The eosinates of methylene azure, methylene violet and methylene blue are dissolved in the methylic alcohol, and the water-soluble products are precipitated and removed by filtration. The materials employed and the quantities are:
The methylic alcohol is put into a 6-ounce bottle, the sodium hydroxid volumetric solution is added, and the two are mixed. To this solution the eosin is added and dissolved. As soon as the eosin is dissolved, the methylene blue is added, and the bottle shaken for thirty minutes. After filtration, the filtrate is put into
Beck FA. METHOD FOR PREPARING THE EOSINATE OF METHYLENE BLUE AND METHOD FOR STAINING. JAMA. 1918;71(20):1651. doi:10.1001/jama.1918.26020460002009c