[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.205.176.107. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Article
December 21, 1918

A SHORT METHOD OF MAKING NOGUCHI ACETONE-INSOLUBLE ANTIGEN

JAMA. 1918;71(25):2056. doi:10.1001/jama.1918.26020510002009a

This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.

Abstract

In order to gain the most information from the Wassermann test, more than one antigen should be used in the "set up." One of the antigens so used should undoubtedly be the

Noguchi acetone-insoluble, as it will give positive reactions with a higher percentage of syphilitic serums than the alcoholic extracts, while it is less likely to give false positive reactions than the more sensitive cholesterinized extracts. But its preparation requires so much time that this becomes quite a factor in a laboratory where considerable quantities are used. With a view of hastening the process of extraction and evaporation we tried this at higher temperatures and find that boiling temperature in no way interferes. In this way we can prepare and titrate an antigen all in one day.

PROCEDURE  To one part of finely minced beef heart, nine parts of commercial ethyl alcohol are added. (We have recently tried the

First Page Preview View Large
First page PDF preview
First page PDF preview
×