In previous articles1 it has been demonstrated that there is present in the nasopharyngeal mucous membrane of patients suffering from epidemic encephalitis a filtrable virus capable of producing in monkeys and rabbits lesions typical of those found in the brain in human cases. By the use of Berkefeld (V and N) filtrates of nasal washings and nasopharyngeal mucous membrane from fatal cases, we were able to develop in monkeys and rabbits the clinical and pathologic pictures of the disease.
In the second article it was stated that cultivation of all material on ordinary laboratory mediums was negative both aerobically and anaerobically. We further stated that the studies by the Noguchi method had been undertaken and were then in progress. We now wish to describe the results of the latter studies on the nasal washings, nasopharyngeal mucous membrane, and brain in both human cases and inoculated animals.
The methods used
LOEWE L, STRAUSS I. ETIOLOGY OF EPIDEMIC (LETHARGIC) ENCEPHALITISPRELIMINARY NOTE. JAMA. 1919;73(14):1056-1057. doi:10.1001/jama.1919.02610400034012