Some years ago one of us (Haines1) introduced a test for sugar in the urine which has found wide acceptance by the profession. The solution suggested at that time contained copper sulphate, potassium hydroxid, glycerin and water in these proportions:
These proportions have been more or less changed by different investigators and transformed into the metric system. The method, used with this test solution, is generally known and consists in heating to boiling about 5 c.c. of the test solution and adding from 8 to 10 drops of the suspected sample of urine. If sugar is present, a brick-red cloud is formed which eventually settles to the bottom of the tube. This solution, if made with pure ingredients, keeps indefinitely and has been found to be relatively delicate and quite reliable.
It occurred to us that the original solution might be improved on in such a way as to
HAINES WS, POND GP, WEBSTER RW. AN IMPROVED TEST FOR THE DETECTION OF GLUCOSE, ESPECIALLY IN URINE. JAMA. 1920;74(5):301-302. doi:10.1001/jama.1920.02620050009003