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This edition comes four years after the first. During these four years so much has been added to the knowledge of stains and staining that it has been necessary largely to rewrite the book. A good deal of new material has been added. Staining formulas and procedures for staining are given in detail under various dyes, but staining methods could not all be included without expanding the book into a complete work on microscopic technic. The procedures outlined in the book are limited to those that have been used by the members of the commission and found to give good results with modern American stains. The investigations of the commission have been supported by the Chemical Foundation. The work of the commission on standardization of biologic stains, as represented in this book, which should be found in all biologic laboratories, is of great practical value.
Biological Stains: A Handbook on the Nature and Uses of the Dyes Employed in the Biological Laboratory.. JAMA. 1930;94(11):817. doi:10.1001/jama.1930.02710370061037