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May 31, 1930


JAMA. 1930;94(22):1773-1774. doi:10.1001/jama.1930.02710480049023

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Measuring the Speed of Circulation  Dr. Lian and Madame E. Barras applied to the study of the measurement of the speed of circulation in man the use of a 5 per cent solution of fluorescein in physiologic solution of sodium chloride. They inject 2 cc. of this solution in a vein of the elbow and then puncture the vein at the same place in both arms. The blood is collected in twelve small glass tubes containing 95 per cent alcohol at 10, 20, 30, 35, 40, 50, 60, 75, 90, 120, 150 and 180 seconds after the injection. Then the tubes are shaken and placed in a. rack for a few minutes. If the tubes are inspected on a dark background, in a room to which no sunlight is admitted, the fluorescence will be readily observed. The speed of circulation in a normal person is thirty seconds between

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