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Fungous disorders of the skin are being seen in such increasing numbers that they are becoming a problem of public health. The first means of prevention is the quick recognition of existing cases. This can most rapidly and conveniently be done by knowing the distinct clinical forms which the different mycoses assume. While, to the expert, clinical means are generally sufficient, he too often needs the aid of the laboratory to be certain of his diagnosis. It is natural, therefore, that improved methods for demonstrating fungi in keratotic structures should be desirable.
There are two laboratory procedures in general use by physicians for examining the skin and hair for fungi. The first is the cultural means and the second is the direct method of maceration with fixed alkalis. The cultural method does not lend itself to routine use by the physician because it requires too much time, material and special
Cornbleet T. A REAGENT FOR DEMONSTRATING FUNGI IN SKIN SCRAPINGS AND HAIR. JAMA. 1930;95(23):1743–1744. doi:10.1001/jama.1930.27210230002010b