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Production of Tetanic Anatoxin
Mr. Ramon, in creating anatoxins, has produced therapeutic anti-infectious agents of a high degree of potency and, at the same time, devoid of harm. His diphtheritic anatoxin is used today throughout France, having replaced completely the serum formerly employed for antidiphtheritic vaccination. By applying the same mode of preparation—the neutralization of the toxin by means of formaldehyde—he has obtained a highly potent tetanic anatoxin. Recent researches, undertaken in collaboration with Dr. G. Lemetayer, have enabled him to increase considerably the production of antitoxin in horses by adding powdered tapioca to the antigen injected into these animals. After this hyperimmunization, which required thirty-five days, he was able to secure serums titrating from 1,200 to 10,000 international units per cubic centimeter. These results are greatly in excess of any that have been reported. There is thus a saving of time and of antigen and a considerable increase in
PARIS. JAMA. 1931;96(18):1519-1521. doi:10.1001/jama.1931.02720440067020