This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.
This report, in Danish, accompanied by an English summary, covers experimental investigations (1929-1931) in which mice were used to ascertain whether requirements for the B vitamins vary at different levels of protein, fat and carbohydrate in the diet. This question is important in the quantitative determination of the B vitamins as well as from a clinical standpoint. When given a rich fat diet, the animals seemed to need less vitamin B1, while the requirement of B2 seemed to be increased; increased vitamin B1 caused an earlier development of pellagra and death. A high carbohydrate diet seemed to increase the need for B1 and/or B4. A high protein diet appeared to increase B2 needs. The results of the experiments show that the composition of the diets on which experimental animals are fed has a large influence on the quantitative biologic determinations of the constituents of
A Contribution to the Problem of the Relationship Between the B Vitamins and the Protein, Fat and Carbohydrate Contents of Food. JAMA. 1934;103(24):1881. doi:10.1001/jama.1934.02750500063035