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The first chapter of this volume is devoted to introductory statements intended for students who lack training in the principles and methods of quantitative analysis. The treatment is extremely elementary and certainly not sufficiently detailed to be of much assistance to the unprepared student. On the other hand, for the prepared student this chapter is superfluous. A chapter on colloids follows and is devoted almost entirely to experiments on inorganic colloidal states. The next three chapters treat carbohydrates, lipins and proteins in an elementary manner. No attempt has been made to present this important qualitative work in a comparative, quantitative manner, and the limitations in the applications of the tests are not discussed. For the quantitative estimation of reducing sugars, one of the oldest and least accurate direct titration methods is emphasized. The chemistry of digestion and the pathologic examination of urine are treated in the same general manner. The
Laboratory Manual of Physiological Chemistry.. JAMA. 1928;91(13):983. doi:10.1001/jama.1928.02700130061036