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September 5, 1931

A MICROSCOPIC METHOD OF TYPING PNEUMOCOCCI BY THE USE OF STAINED ORGANISMS

JAMA. 1931;97(10):698-700. doi:10.1001/jama.1931.02730100022008
Abstract

As the success of serum treatment in lobar pneumonia depends largely on its administration early in the course of the disease, the importance of speed in typing the offending organism becomes apparent immediately, and any method designed to hasten the process of typing merits publication.

The method here presented, which makes use of hanging drop preparations of stained organisms, has been applied to thirty-eight different strains obtained from the following sources: two cases of pneumococcus meningitis; two cases of pneumococcus empyema; twenty-six cases of lobar pneumonia and eight laboratory stock strains. The distribution of the various types was as follows: type I, ten cases; type II, eight cases; type III, six cases; type IV, one case; type V, two cases; type VI, one case; type VII, two cases; type VIII, two cases; and group IV, sub-type undetermined, six cases. In every instance, organisms obtained both from Avery's medium (dextrose-blood-broth, p

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