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Article
April 8, 1944

FROZEN-DRIED NERVE GRAFTS

JAMA. 1944;124(15):1063-1064. doi:10.1001/jama.1944.02850150043015
Abstract

Supplementing his earlier experiments on rats, Paul Weiss1 of the Department of Zoology, University of Chicago, has developed a successful technic for the transplantation of stored frozen-dried nerve grafts into cats, monkeys and other larger animals,2 a technic presumably applicable to man. In order to avoid sacrificing a "minor" nerve for the repair of a "more vital" one, earlier experimenters tested the feasibility of transplanting stored, preserved or fixed nerve tissues. Most of these attempts were unsuccessful, presumably because of autolysis or other forms of denaturation of stored nerve segments. Weiss tried the method of immediately freezing and dehydrating the excised nerve segments, a method of preservation and storage used with success in other fields of biochemical research.3 Nerves dissected aseptically were dropped into isopentane immersed in liquid nitrogen (— 195 C.), where they were frozen instantaneously. The frozen nerve segments were then dehydrated for one week

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