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February 27, 1937

FACTORS IN THE GROWTH AND IDENTIFICATION OF THE GONOCOCCUS

Author Affiliations

Chicago. Resident in Pathology, Cook County Hospital.

JAMA. 1937;108(9):746. doi:10.1001/jama.1937.02780090058022

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Abstract

To the Editor:—  I have read with interest in Current Comment (The Journal, Nov. 7, 1936, p. 1564) the paragraph entitled "Cultural Methods for the Diagnosis of Gonococcic Infections." In the course of several years' study I have had an opportunity to compare the various mediums recommended for the growth of the gonococcus. Included in two papers (Proc. Soc. Exper. Biol. & Med.31:899 [May] 1934 and J. Infect. Dis.55:328 [Nov.-Dec.] 1934) is the description of what proved a most efficient medium. This was a modification of Hitchen's semisolid agar, which differs from the standard mediums in that it contains potassium nitrate (0.2 per cent) instead of sodium chloride (0.5 per cent). Enrichment fluids were added to the medium used for plating and these included whole blood, egg yolk, serum and transudates, each with good result. However, ascitic fluid was found more practical since it was easily

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