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This is a conservative laboratory manual, including simple experiments in physical chemistry, quantitative tests of the elements in the body, and simple experiments on proteins, fats and carbohydrates, lipids, foods, digestion, blood and urine. The book is modernized to the extent that the chemical balance is not used but, on the other hand, no use is made of a colorimeter, although an attempt is made to perform quantitative colorimetric comparisons in a test tube. This limits the use of quantitative micromethods to sugar, hemoglobin and urea on blood. Some quantitative work is done on the urine. Although the book contains few errors, some statements might mislead a student. Thus the xanthoproteic reaction is mentioned in connection with phenylalanine, whereas this reaction is so slow on phenylalanine that it is usually considered negative. The only quantitative determination of protein is the Sörenson titration for amino nitrogen in milk. The number of
Practical Physiological Chemistry for Medical Students. JAMA. 1937;108(12):1000. doi:10.1001/jama.1937.02780120070032