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Article
February 27, 1932

HENDERSON IMPROVED STAIN FOR STIPPLE CELLS

Author Affiliations

Pennsgrove, N. J.

JAMA. 1932;98(9):733. doi:10.1001/jama.1932.27320350001011

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Abstract

To those interested in blood work in connection with the lead industry, I wish to suggest the use of a stain for stipple cells that has proved superior to all others that have been used in an industrial hospital with which I am associated.

This stain was developed by Carl W. Henderson, laboratory technician, du Pont Dye Works Hospital. He examines an average of ninety smears a week and with the usual method of staining too much time was required. With the Henderson method only about one-half minute is required, as compared to five minutes with other stains.

There is no precipitate, making it easier, therefore, to distinguish the basophilic granular cells. The contrast is greater and there is only one step in staining the smear.

Blood smears do not need to be fixed. A Coplin jar is used. Smears are placed in the Coplin staining jar of solution for

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