This investigation of patients with amenorrhea is a continuation of our studies on oligomenorrhea, amenorrhea and sterility begun in 1931.1 Since our last publications the methods for determining the gonadotropic factors in both blood and urine have been improved.2 As far as treatment is concerned, pure estrogenic substances are now available in large dosage. In addition to gonadotropic substances from pregnancy urine or the placenta, which lack some of the properties of the adenohypophysis, physiologically potent gland preparations and their equivalent—the serum of pregnant mares—are now available. This permits a better evaluation of any prepituitary effects that might be obtained in our carefully controlled series of cases.
The patients selected had suffered with amenorrhea for various periods and were of various ages. Intelligence, faithful carrying out of orders, ability and willingness to report as directed were of importance in the choice. In one instance the investigation covered
FRANK RT, GOLDBERGER MA, SALMON UJ, FELSHIN G. AMENORRHEA: ITS CAUSATION AND TREATMENT. JAMA. 1937;109(23):1863-1869. doi:10.1001/jama.1937.02780490001001