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For fifty years Bolles Lee's book has been widely used in histologic and embryologic laboratories. It continues to be a standard and convenient reference work for the research laboratory. The present editors have tried to widen its scope by adding a brief outline of technical procedures, but the encyclopedic character of the work makes it unsuitable for beginners especially as there are several good elementary manuals available. Bolles Lee personally tested every method he thought worth including in his book. Dr. Gatenby finds this impracticable at the present time, so many new methods have been accepted on the authority of those who have described them. On the other hand he has added critical discussions of certain cytologic problems in fixation and staining which should clarify the situation. There are still many biologists who believe that staining methods have the specificity of qualitative chemical tests. A careful reading of these discussions
The Microtomist's Vade-Mecum (Bolles Lee): A Handbook of the Methods of Animal and Plant Microscopic Anatomy. JAMA. 1938;110(16):1311. doi:10.1001/jama.1938.02790160069027