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April 13, 1946


JAMA. 1946;130(15):1017-1018. doi:10.1001/jama.1946.02870150035010

Supplementing their earlier work with meningococcus endotoxin1 Miller and his associates2 of the University of Chicago Department of Medicine report that penicillin has an equally effective neutralizing action against gonococcus endotoxin. The endotoxin used in their tests was prepared from five strains of gonococcus grown for eighteen hours on an agar medium containing casein digest and cystine. The organisms were repeatedly washed in saline solution, suspended in distilled water and then allowed to autolyze for six hours at room temperature, followed by fourteen hours' storage at 4 C. The autolysate was then neutralized, heat sterilized (60 C. for thirty minutes) and its concentration adjusted to about 1 per cent solids. White mice weighing 18 to 20 Gm. were injected intraperitoneally with graded doses (0.1 to 0.5 cc.) of this 1 per cent endotoxin. Half of each group served as untreated controls. The other half were each given eight

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