Mumps as a disease of military personnel may be exceedingly troublesome, causing loss of time from duty and interfering with the movement of troops. Extensive investigations were therefore undertaken by Enders and his associates1 of Harvard Medical School in hope of developing specific diagnostic methods for doubtful cases and the discovery of a successful prophylactic vaccine.
In their initial experiments about 3 cc. of saliva collected on the first and second days of the disease in a typical human attack was introduced into the Stensen duct of a monkey (Macacus mulatta). On the fifth day the gland, which was somewhat enlarged, was removed, emulsified and inoculated into both ducts of a second monkey. On the seventh day the second animal presented swelling of both parotid glands with subcutaneous edema of both cheeks. In this way the virus was passed through a series of monkeys, some of which received bacteriologically
IMPROVED MUMPS VACCINE. JAMA. 1947;133(13):939. doi:10.1001/jama.1947.02880130039012