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The first edition of this book appeared in 1934. Thus, in 20 years six editions have appeared and several editions have had to be reprinted; from this fact alone one may judge the value of this book. Although Koch and Hanke are listed as authors, this edition is the work of the latter, since Dr. Koch died in 1948. This edition maintains the excellent quality exhibited by the earlier editions and has kept the information abreast of the newer techniques by the addition of new experiments, including the paper chromatographic and ion exchange techniques for separation and identification of amino acids, studies in enzyme kinetics, manometric study of succinic dehydrogenase, the use of diethylene glycol as a solvent for the determination of saponification numbers, the estimation of pepsin by the hemoglobin method, and the microbiological determination of methionine and cobalamine. There are many other changes and some rearrangement of experiments
Practical Methods in Biochemistry.. JAMA. 1954;155(11):1018. doi:10.1001/jama.1954.03690290068029