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November 2, 1940

IMPROVED METHOD OF STAINING WITHIN TISSUES, LEPTOTRICHES OF PARINAUD'S CONJUNCTIVITIS AND GRAM-POSITIVE MICRO-ORGANISMS

Author Affiliations

Boston

From the Howe Laboratory of Ophthalmology, Harvard Medical School, and the Massachusetts Eye and Ear Infirmary.

JAMA. 1940;115(18):1546-1547. doi:10.1001/jama.1940.72810440003008a
Abstract

Judging by my own experience, it is always possible by means of the staining method1 described by me in 1913 to demonstrate leptotriches in any section which contains one or more of the characteristic foci of leptotrichosis conjunctivae2 (Parinaud's conjunctivitis). Assuming that this disease is as frequent in many other localities as it is in Boston (two or three cases a year), failure by other investigators to find these micro-organisms has often been due to the employment of faulty technic. Judging by sections that have been sent to me, a common error is failure to exercise sufficient care in making the biopsy. Only a small bit of tissue need be excised, but since the leptotriches can be readily found only in the foci of macrophages it is essential to include at least one of the gray areas, preferably the largest, that can be seen just beneath the surface

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