The conversion of ingested carbohydrate into fat in animals and man is a commonly recognized and extremely useful phenomenon; the reverse reaction, on the other hand, had been debated for many years, and definite information regarding this transformation would be of great physiological significance. Using the long-lived radioactive isotope C14, Strisower, Chaikoff and Weinman1 have studied the conversion of the long chain palmitic acid into glucose by rats rendered diabetic with alloxan. The fatty acid used in these studies was labeled in either the 1 or the 6 position and was converted into the neutral fat tripalmitin before testing.
Two types of studies were carried out to measure the conversion of the labeled fatty acid into glucose. One was based on the recovery of the radioactive carbon in the glucose excreted by the diabetic rats in 24 hours, whereas the second depended on the amount of radioactive carbon
CONVERSION OF PALMITIC ACID TO GLUCOSE. JAMA. 1951;147(18):1766. doi:10.1001/jama.1951.03670350046017