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February 2, 1952


JAMA. 1952;148(5):376. doi:10.1001/jama.1952.02930050048014

Over a period of years evidence that the liver is the site of formation of plasma albumin, fibrinogen, and, possibly, globulin fractions has accumulated from both experimental procedures and observations in pathological states. Recently, Miller and associates1 reinvestigated this question using newly developed techniques. They perfused isolated, intact rat livers with rat blood containing isotopically labelled lysine together with the amino acids essential for optimum growth in the rat and subsequently determined the rate of incorporation of radioactive amino acid in the liver and in plasma proteins. In contrast to experimental devices employing tissue slices or homogenates, the isolated liver still remains active metabolically as long as four hours after the initiation of the perfusion, and studies could be continued for about six hours. The perfused liver was able to differentiate between the L- or natural isomer and the D- or unnatural isomer of C14-labelled lysine, the

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