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This series of volumes is intended to provide ready accessibility of reliable detailed information on working methods to be used in research. The fourth volume is divided into four sections, each of which represents a major field in medical research. The first deals with histochemical staining methods for the presence of various inorganic elements and certain organic substances including the oxidative and hydrolytic enzymes. The second section concerns itself with fluid and electrolyte distribution. The methods for estimation of the volume of the body fluids as well as flame photometer techniques are described. The third division considers problems relative to digestive tract pressures, innervation, and secretions. The last section describes tissue culture techniques, including the media used and cytochemical studies. A copious bibliography accompanies each section. The editors are to be congratulated, both for the concept and its execution in this volume. The text is solid, with little extraneous matter,
Methods in Medical Research. Volume 4. JAMA. 1952;148(11):972. doi:10.1001/jama.1952.02930110094031