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Article
July 26, 1958

EVALUATION OF A NITROPRUSSIDE DIP TEST FOR KETONE BODIES

Author Affiliations

Chicago

Attending Physician, Grant Hospital of Chicago, and Clinical Instructor, Department of Medicine, University of Illinois College of Medicine (Dr. Chertack), and Pathologist, Grant Hospital of Chicago, and Clinical Assistant Professor of Pathology, University of Illinois College of Medicine (Dr. Sherrick).

JAMA. 1958;167(13):1621-1624. doi:10.1001/jama.1958.72990300004010b
Abstract

The detection of ketone bodies in urine specimens is sufficiently important to warrant the inclusion of a nitroprusside test for ketonuria as a part of the routine urinalysis. In the past, methods available have included a tablet test (Acetest) and the Rothera test, a conventional test tube procedure. Recently the nitroprusside test for urine ketones has been made available as a stick or dip test with a preparation called Ketostix. This test, like the other nitroprusside tests, depends on the reaction between acetoacetic acid and nitroprusside to produce a colored complex. In order to determine the accuracy and simplicity of the new test, it was compared with the two more standard procedures on a series of routine urine specimens in a general hospital laboratory. Tests were also carried out by experienced and inexperienced personnel on urines with and without added acetoacetic acid. The present report describes the results obtained in

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