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Article
July 3, 1967

Screening Tests for BacteriuriaA Validity Study

Author Affiliations

From the departments of clinical microbiology and social medicine, Hebrew University-Hadassah Medical School, and the Hadassah University Hospital, Jerusalem, Israel.

JAMA. 1967;201(1):1-4. doi:10.1001/jama.1967.03130010027005
Abstract

An evaluation of numerous reported studies indicated that the most useful screening tests for significant bacteriuria appear to be a nitrite test (modified by adding nitrate and incubating at 37 C), the triphenyltetrazolium chloride (TTC) test, and microscopic examination for bacteria. A study of 1,151 urine specimens submitted for routine culture showed that microscopic examination of the centrifuged deposit for organisms or pus cells, or both, was a more sensitive test for significant bacteriuria than the modified nitrite test. Microscopic examination detected 98% of 175 urine specimens with significant gram negative bacilluria, and 17 of 20 with significant numbers of gram positive cocci. The false positive rate was 13%. Microscopy may be better than the chemical screening tests in the selection of urine specimens for cultural examination.

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