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Article
May 12, 1975

Staining of Iron

Author Affiliations

Los Angeles

JAMA. 1975;232(6):595. doi:10.1001/jama.1975.03250060011005
Abstract

To the Editor.—  Bark's query (231:25, 1975) about the failure of decalcified marrow to react with the Prussian blue stain for iron is answered by Schmorl.1 In the fixation for pigment identification, Schmorl warns against the use of acid fixatives if search for iron-containing pigments is planned. Hence the recommendation for the use of many fixatives. Hemosiderin, for example, "is soluble in acids, insoluble in alkalis and fat-dissolving media, is not attacked by bleaches and is not blackened either by osmic acid or silver nitrate."1(p183)

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