July 14, 1975

Identification of Enterococci

Author Affiliations

Peninsula General Hospital Salisbury, Md

JAMA. 1975;233(2):136. doi:10.1001/jama.1975.03260020022005

To the Editor.—  The report by Neefe et al on Streptococcus mutans endocarditis (230:1298-1299,1974) refers to two media employed for the differentiation of enterococci, S faecalis broth and Enterococcosel broth, and indicates that some strains of S mutans can also grow in these media. In addition to these two broths, several other media are available for the differentiation of group D streptococci or enterococci. As indicated in the Table, each of these media is unique in the amount of either bile or esculin present. In addition, all of the media except Bile Esculin Agar also contain sodium azide. Since these three ingredients are the major selective and differential components of the media, it is important to keep these differences in mind when using these media in any identification scheme.In a much larger survey than that of Neefe et al, Facklam and his colleagues1 have evaluated various alternative biochemical