Sixteen patients receiving long-term hemodialysis therapy demonstrated a decline in leukocyte ascorbic acid concentration of 26% during a single hemodialysis. Eight patients receiving 1 gm of ascorbic acid by mouth showed a 76% rise in leukocyte ascorbic acid concentration in three hours, while control subjects showed a 5% increase. When ascorbic acid was added to dialysate baths of 12 patients, plasma ascorbate levels were elevated by 26% at the end of dialysis, and leukocyte levels rose by 11%. Short-term hemodialysis therapy over five consecutive days produced a continuous decline in plasma and leukocyte ascorbate levels. Ascorbic acid loss from leukocytes and plasma during hemodialysis can be prevented by the addition of the vitamin to dialysate concentrate.
Sullivan JF, Eisenstein AB. Ascorbic Acid Depletion During Hemodialysis. JAMA. 1972;220(13):1697-1699. doi:10.1001/jama.1972.03200130029006