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Burkhardt has led in developing a system of obtaining and examining sections incorporating cortex, bone spicules, and bone marrow from the iliac crest. This system guarantees an ample, undistorted piece of tissue. It utilizes newer techniques of fixation, sectioning, and staining, so that one may readily study both histology and cytology of the bone and marrow; but it involves more of a surgical procedure, special processing equipment, and a considerably longer period for specimen preparation than does marrow aspiration. The author believes that his system produces material for study superior to those obtained by marrow aspiration and the generally used methods for marrow section. This volume is his chef d'oeuvre for promoting his system and for guiding those who would use it.
The technique involves removing a cylinder of bone and marrow about 4 × 18 mm. After special embedding it is cut into serial sections of 1μ to 3μ
Best WR. Bone Marrow and Bone Tissue: Color Atlas of Clinical Histopathology. JAMA. 1972;221(8):920. doi:10.1001/jama.1972.03200210064031