MS-1 serum, previously shown to produce hepatitis in infants, produced hepatitis in three of ten adult male volunteers when 0.05 ml of infectious serum was introduced orally. One plasma sample, taken before the period of rising transaminase levels from one of the volunteers who developed hepatitis, did not produce hepatitis when passed to ten volunteers. However, plasma obtained at the time of maximum transaminase levels from another of the men produced hepatitis in five of ten volunteers. When acute-phase plasma from two of the volunteers was inoculated and passed in a serially cloned line of Detroit-6 cells, cytopathic effects occurred. No cytopathogenic agents were present in the plasma of the same volunteers before inoculation, or in the cell controls carried in parallel with each passage. These findings call for continued work with hepatitis virus in tissue culture.
Boggs JD, Melnick JL, Conrad ME, Felsher BF. Viral HepatitisClinical and Tissue Culture Studies. JAMA. 1970;214(6):1041–1046. doi:10.1001/jama.1970.03180060019004