April 11, 1986

Stability and Inactivation of HTLV-III/LAV Under Clinical and Laboratory Environments

Author Affiliations

From the Laboratory of Tumor Cell Biology, National Institutes of Health, Bethesda, Md (Dr Resnick and Mr Salahuddin); the Department of Cell Biology, Bionetics Research, Inc, Rockville, Md (Mr Veren, Ms Tondreau, and Dr Markham); and the Dermatology Department, Mount Sinai Hospital-Medical Center, Miami Beach, Fla (Dr Resnick).

JAMA. 1986;255(14):1887-1891. doi:10.1001/jama.1986.03370140085029

The stability of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) under environmental conditions encountered in a clinical or laboratory setting and its inactivation by commonly used chemical disinfectants were investigated. Under our experimental conditions utilizing a highly concentrated viral preparation, virus with an initial infectious titer of approximately 7 log10 tissue culture infectious dose (TCID50) per milliliter can be recovered for more than a week from an aqueous environment held at room temperature (23 to 27 °C) or at 36 to 37 °C. Virus recovery is reduced at a rate of approximately 1 log10TCID50 per 20 minutes when held at 54 to 56 °C. Dried and held at room temperature, HTLV-III/LAV retains infectivity for more than three days with a reduction of approximately 1 log10TCID50 per nine hours. Viral infectivity is undetectable and reduced more than 7 log10TCID50 within one minute with 0.5% sodium hypochlorite, 70% alcohol, or 0.5% nonidet-P40, and within ten minutes with 0.08% quaternary ammonium chloride or with a 1:1 mixture of acetone-alcohol. These results help provide a rational basis to prevent the accidental spread of HTLV-III/LAV in the laboratory or clinical setting.

(JAMA 1986;255:1887-1891)