Since many have failed to cultivate the cause of trachoma, it seemed best to try special media. I had been doing some cultivation of the Koch-Weeks bacillus on 5 per cent. peptone, alkaline agar, and decided to try that same composition for the cause of trachoma, but, having in mind the fact that the gonococcus grows only in the presence of fresh blood or some exudate of the body, I added a sterile drop of blood from the ear of the patient from whom we tried to get the culture. This drop was smeared on the surface of the agar with a sterile platinum needle. At least one tube containing no attempt at culture was set aside in each case as a control of the securing of the blood in a sterile condition.
As is seen, two of the four cases examined were negative culturally, and a third gave a very
EDWARDS RT. TRACHOMACULTIVATION OF A NEW BACILLUS. JAMA. 1910;LIV(12):965–966. doi:10.1001/jama.1910.92550380001001p