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September 21, 1912

SUCCESSFUL CULTIVATION OF MALARIAI PLASMODIA

Author Affiliations

Assistant Professor of Tropical Medicine, Medical Department of the Tulane University of Louisiana NEW ORLEANS

JAMA. 1912;LIX(12):936. doi:10.1001/jama.1912.04270090180007
Abstract

In a former contribution1 I first reported my success in cultivating Plasmodium vivax, P. malariœ and P. falciparum; and as much additional work has been completed since the appearance of that article, the present brief announcement of results to date is presented pending publication of the full technic and details.2

The work subsequent to that first described in the article above cited1 was done in Central America in conjunction with Dr. Foster M. Johns on a research expedition equipped and sent to Panama by the Tulane School of Tropical Medicine and Hygiene.

In the course of this later work it was found that the malarial plasmodia can be grown in human serum, in Locke's fluid (from which calcium chlorid is omitted) and in human ascitic fluid. In the majority of cases it was found that dextrose must be added to the medium in order to secure growth of the

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