These studies were undertaken for the purpose of further investigating certain reactions on which Klotz1 bases in large part his interpretation of pathologic calcification and which, as his interpretation is not in accord with that reached by Wells2 and independently by me,3 are matters of considerable importance in the elucidation of this interesting problem.
The observations in question are those concerning the value of the so-called proteid-soap compounds and of the staining of fats and lipoids by Sudan III and Scharlach R.
Klotz, as the result of a series of microchemical examinations of various kinds of calcified tissues, concluded that the deposition of lime in tissues undergoing calcareous degeneration is dependent on the formation of calcium soaps as an intermediate step. He further states that an essential factor in the production of these soaps is the initial combination of a soluble soap and an albuminous
BALDAUF LK. AN INVESTIGATION OF THE NATURE OF PROTEID-SOAP COMPOUNDS AND OF THE STAINING OF PURE FATS AND LIPOIDS BY SCHARLACH R AND SUDAN III.. JAMA. 1907;XLIX(8):642-647. doi:10.1001/jama.1907.25320080010001c